Inmunofijación en suero
Resumen
La inmunofijación en suero consiste en la detección, mediante electroforesis alcalina en gel de agarosa y uso de anticuerpos, de proteínas monoclonales en suero. Las proteínas migran en la electroforesis y se inmunofijan con antisueros de diferentes especificidades: anti-gamma, para la detección de inmunoglobulina G (IgG); anti-alfa, para inmunoglobulina A (IgA); anti-mu, para inmunoglobulina M (IgM); anti-lambda, para cadenas ligeras lambda, y anti-kappa, para cadenas kappa. Posterior a la inmunofijación, las proteínas que se precipitan se tiñen con violeta ácido para permitir su visualización.
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Referencias bibliográficas
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https://doi.org/10.1093/clinchem/48.9.1437
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https://doi.org/10.1128/CVI.13.2.277-280.2006
2. Bird JM, Owen RG, D'Sa S, Snowden JA, Pratt G, Ashcroft J, et al. Guidelines for the diagnosis and management of multiple myeloma 2011. Br J Haematol 2011; 154: 32-75.
https://doi.org/10.1111/j.1365-2141.2011.08573.x
3. Munshi NC. Investigative tools for diagnosis and management. Hematology Am Soc Hematol Educ Program 2008: 298-305.
https://doi.org/10.1182/asheducation-2008.1.298
4. Durie BG, Harousseau JL, Miguel JS, Blade J, Barlogie B, Anderson K, et al. International uniform response criteria for multiple myeloma. Leukemia 2006; 20: 1467-1473.
https://doi.org/10.1038/sj.leu.2404284
5. Kyle RA, Rajkumar SV. Criteria for diagnosis, staging, risk stratification and response assessment of multiple myeloma. Leukemia 2009; 23: 3-9.
https://doi.org/10.1038/leu.2008.291
6. Jaffe ES, Harris NL, Vardiman JW, Campo E, Arber DA. Hematopathology. Philadelphia: Elsevier Saunders; 2011.
7. Feller AC, Diebold J. Histopathology of Nodal and Extranodal Non-Hodgkin's Lymphomas (ed 3rd). Berlin: Springer-Verlag; 2004.
https://doi.org/10.1007/978-3-642-18653-0
8. Katzmann JA, Clark RJ, Abraham RS, Bryant S, Lymp JF, Bradwell AR, et al. Serum reference intervals and diagnostic ranges for free kappa and free lambda immunoglobulin light chains: relative sensitivity for detection of monoclonal light chains. Clin Chem 2002; 48: 1437-1444.
https://doi.org/10.1093/clinchem/48.9.1437
9. Jaskowski TD, Litwin CM, Hill HR. Detection of kappa and lambda light chain monoclonal proteins in human serum: automated immunoassay versus immunofixation electrophoresis. Clin Vaccine Immunol 2006; 13: 277-280.
https://doi.org/10.1128/CVI.13.2.277-280.2006
Cómo citar
1.
Medicina & Laboratorio. Inmunofijación en suero. Med. Lab. [Internet]. 1 de julio de 2013 [citado 21 de marzo de 2023];19(7-8):395-8. Disponible en: https://medicinaylaboratorio.com/index.php/myl/article/view/239
