Histoquímica enzimática en el diagnóstico de miopatías: revisión sistemática

  • Doris H. Rosero-Salazar Universidad del valle
  • Liliana Salazar-Monsalve Universidad del Valle
  • María A. Tovar-Sánchez Universidad del Valle
Palabras clave: NAD, músculo esquelético, distrofias musculares, miosinas, miopatía, inmunohistoquímica.

Resumen

Introducción: Las enfermedades del músculo esquelético en las que la lesión supera la capacidad de adaptación conducen a cambios patológicos propios de las miopatías. En Colombia el diagnóstico se realiza mediante tinción con hematoxilina-eosina; sin embargo, la información brindada sobre algunos de los cambios del tejido muscular es limitada, por lo que es necesario realizar algunas técnicas de histoquímica enzimática según la alteración. Objetivo: Describir los criterios de tinción de diferentes técnicas histoquímicas en el diagnóstico de las miopatías. Materiales y métodos: Se realizó una revisión sistemática mediante la búsqueda de artículos en inglés y español indexados en las bases de ISI Web of Science, Ovid Medline, PubMed, ScienceDirect y EBSCO. Los descriptores MeSH utilizados fueron: “histochemistry”, “skeletal muscle”, “structural and congenital myopathies”, “fiber type fast twitch”, “fiber type slow twitch” y “NADH tetrazolium reductase”. Se asociaron a la búsqueda los términos “clinical application” y “diagnosis”. Se seleccionaron los artículos originales, reportes de casos y revisiones publicadas entre 1982 y 2014 que aplicaran las técnicas de histoquímica en el diagnóstico de una miopatía o en la investigación biomédica. Resultados: Se seleccionaron 64 publicaciones (incluidos dos libros) que fueron representativos para la redacción de este manuscrito, el cual fue complementado con imágenes de biopsias musculares procesadas en el Laboratorio de Histología de la Universidad del Valle, Colombia. Conclusiones: Un protocolo estandarizado con técnicas de histoquímica convencional y enzimática, asociado a los criterios patológicos, permite orientar el diagnóstico de las miopatías oportunamente y a un menor costo respecto a otras técnicas disponibles.

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Biografía del autor/a

Doris H. Rosero-Salazar, Universidad del valle

Enfermera, MSc en Ciencias Biomédicas. Grupo de investigación Tejidos Blandos y Mineralizados, Departamento de Morfología, Universidad del Valle. Cali, Colombia.

Liliana Salazar-Monsalve, Universidad del Valle

Fisioterapeuta, MSc en Morfología. Grupo de investigación Tejidos Blandos y Mineralizados, Departamento de Morfología, Universidad del Valle. Cali, Colombia.

María A. Tovar-Sánchez , Universidad del Valle

Médica, cirujana, especialista en Medicina Física y Rehabilitación, especialista en Docencia Universitaria. Departamento de Medicina Física y Rehabilitación, Universidad del Valle. Cali, Colombia.

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Cómo citar
1.
Rosero-Salazar DH, Salazar-Monsalve L, Tovar-Sánchez MA. Histoquímica enzimática en el diagnóstico de miopatías: revisión sistemática. Med. Lab. [Internet]. 1 de septiembre de 2014 [citado 5 de octubre de 2022];20(9-10):467-88. Disponible en: https://medicinaylaboratorio.com/index.php/myl/article/view/195
Publicado
2014-09-01
Sección
Tecnología