La calidad espermática evaluada mediante metodologías no convencionales

  • Jenniffer Puerta-Suárez Universidad de Antioquia
  • José Manuel Mayorga-Torres Universidad de Antioquia
  • Luisa Ospina-Medina Universidad de Antioquia
  • Jesús Berdugo-Gutiérrez Universidad de Antioquia
  • Walter Cardona-Maya Universidad de Antioquia
Palabras clave: análisis de semen, movilidad espermática, viabilidad espermática, cabeza del espermatozoide, cola del espermatozoide, pieza intermedia del espermatozoide.

Resumen

Existen diferentes metodologías para evaluar la calidad seminal, siendo la valoración de la movilidad y de la morfología espermática los indicadores más comúnmente utilizados, sin embargo, los espermatozoides poseen ciertas características que no siempre pueden analizarse a través del examen tradicional. En esta revisión de la literatura se describen algunas metodologías alternativas empleadas para observar y evaluar las características seminales. La movilidad, la viabilidad y la morfología espermática pueden evaluarse empleando metodologías manuales y análisis asistidos por computador. Otras características evaluables de la biología espermática son la producción de especies reactivas del oxígeno, la calidad mitocondrial y el ADN espermático. Esta revisión demuestra que existe una amplia disponibilidad de metodologías para el análisis seminal, sin embargo, cada día se siguen implementando nuevas técnicas, lo que impactará en el entendimiento de la fisiología espermática. En un futuro estas herramientas diagnósticas podrán incidir en el beneficio de los pacientes con infertilidad.

Descargas

La descarga de datos todavía no está disponible.

Biografía del autor/a

Jenniffer Puerta-Suárez, Universidad de Antioquia

Microbióloga, Grupo Reproducción, Facultad de Medicina, Universidad de Antioquia. Medellín, Colombia.

José Manuel Mayorga-Torres, Universidad de Antioquia

Biólogo, Grupo Reproducción, Facultad de Medicina, Universidad de Antioquia. Medellín, Colombia.

Luisa Ospina-Medina, Universidad de Antioquia

Microbióloga, Grupo Reproducción, Facultad de Medicina, Universidad de Antioquia. Medellín, Colombia.

Jesús Berdugo-Gutiérrez, Universidad de Antioquia

Médico Veterinario, MSc en Genética humana, Grupo Reproducción, Facultad de Medicina, Universidad de Antioquia, Medellín, Colombia.

Walter Cardona-Maya, Universidad de Antioquia

Bacteriólogo, MSc en Ciencias Básicas Biomédicas, PhD en Biología. Grupo Reproducción, Facultad de Medicina, Universidad de Antioquia, Medellín, Colombia.

Referencias bibliográficas

World Health Organization. 2010. Laboratory Manual for the Examination and Processing of Human Semen. Fifth Edition.

Abu Hassan Abu D, Franken DR, Hoffman B, Henkel R. Accurate sperm morphology assessment predicts sperm function. Andrologia 2012; 44 Suppl 1: 571-577.

https://doi.org/10.1111/j.1439-0272.2011.01229.x

Cardona Maya WD, Berdugo Gutierrez JA, de los Rios J, Cadavid Jaramillo AP. Functional evaluation of sperm in Colombian fertile men. Arch Esp Urol 2007; 60: 827-831.

https://doi.org/10.4321/S0004-06142007000700019

Smikle CB, Turek PJ. Hypo-osmotic swelling can accurately assess the viability of nonmotile sperm. Mol Reprod Dev 1997; 47: 200-203.

https://doi.org/10.1002/(SICI)1098-2795(199706)47:2<200::AID-MRD11>3.0.CO;2-3

Molecular Probes Inc. LIVE/DEAD® Sperm Viability Kit Eugene, Oregon, USA: Molecular Probes, Inc.; 2001.

Nasr-Esfahani MH, Aboutorabi R, Esfandiari E, Mardani M. Sperm MTT viability assay: a new method for evaluation of human sperm viability. J Assist Reprod Genet 2002; 19: 477-482.

https://doi.org/10.1023/A:1020310503143

Agarwal A, Allamaneni SS, Said TM. Chemiluminescence technique for measuring reactive oxygen species. Reprod Biomed Online 2004; 9: 466-468.

https://doi.org/10.1016/S1472-6483(10)61284-9

Agarwal A, Said TM. Sperm chromatin assessment In: Gardner DK, Weissman A, Howles CM, Shoham Z, eds. Textbook of Assisted Reproductive Techniques (ed 2da). Londres, Reino Unido: Taylor & Francis Ltd; 2005: 93-106.

Gil-Villa AM, Cardona-Maya W, Agarwal A, Sharma R, Cadavid Á. Assessment of sperm factors possibly involved in early recurrent pregnancy loss. Fertil Steril 2010; 94: 1465-1472.

https://doi.org/10.1016/j.fertnstert.2009.05.042

Roa Guerrero E, Cortés Mancera F, Guerrero González N, Cardona Maya W, Morantes Guzmán L. Evaluación asistida por computador de la viabilidad espermática en humanos. Rev Ing Biomed 2012; 6.

Mayorga-Torres BJ, Cardona-Maya W, Cadavid A, Camargo M. Evaluación de los parámetros funcionales espermáticos en individuos infértiles normozooespérmicos. Actas Urol Esp 2013; 37: 221-227.

https://doi.org/10.1016/j.acuro.2012.06.008

de los Rios J, Cardona-Maya W, Berdugo JA, Correa C, Arenas A, Olivera-Angel M, et al. Los valores espermáticos de 113 individuos con fertilidad reciente no mostraron correlación con los parámetros establecidos por la OMS. Arch Esp Urol 2004; 57: 147-152.

Berdugo J, Andrade-Rocha F, Cardona-Maya W. Parámetros seminales en hombres fértiles de dos poblaciones sudamericanas. Arch Esp Urol 2009; 62: 646-650.

https://doi.org/10.4321/S0004-06142009000800006

Rodríguez E, Gil-Villa AM, Aguirre-Acevedo DC, Cardona-Maya W, Cadavid ÁP. Evaluación de parámetros seminales no convencionales en individuos cuyas parejas presentan muerte embrionaria temprana recurrente: en busca de un valor de referencia. Biomédica 2011; 31: 100-107.

https://doi.org/10.7705/biomedica.v31i1.340

Cardona Maya W. Publications about sperm during the years 1897 to 2010. Med J Reprod Infertil 2011; 12: 43.

Cardona Maya WD, Cadavid Jaramillo AP. Complementariedad intergametos: breve revisión. Arch Esp Urol 2004; 57: 1107-1112.

Aulesa C, Cabrera M, Alonso R, Benítez M, Martínez M. Evaluación del sistema automatizado Sperm Class Analyzer® (SCA) para análisis del semen. Revdel Lab Clin 2009; 2: 8-16.

https://doi.org/10.1016/j.labcli.2008.09.001

Chen CS, Chao HT, Leng CH, Pan RL, Wei YH. Direct measurement of the tail beat frequency of human sperm by flash light synchronization. Andrologia 1998; 30: 49-54.

https://doi.org/10.1111/j.1439-0272.1998.tb01382.x

Nascimento JL, Botvinick EL, Shi LZ, Durrant B, Berns MW. Analysis of sperm motility using optical tweezers. J Biomed Opt 2006; 11: 044001-044001-044008.

https://doi.org/10.1117/1.2337559

Nascimento JM. Analysis of sperm motility and physiology using optical tweezers. Electrical Engineering (Photonics). Vol. Ph.D. San Diego, California, USA: University of California; 2008: 187.

McCormack MC, McCallum S, Behr B. A novel microfluidic device for male subfertility screening. J Urol 2006; 175: 2223-2227.

https://doi.org/10.1016/S0022-5347(06)00276-X

Su TW, Xue L, Ozcan A. High-throughput lensfree 3D tracking of human sperms reveals rare statistics of helical trajectories. Proc Natl Acad Sci U S A 2012; 109: 16018-16022.

https://doi.org/10.1073/pnas.1212506109

Cardona-Maya W. Words of wisdom: re: high-throughput lensfree 3D tracking of human sperms reveals rare statistics of helical trajectories. Eur Urol 2013; 63: 768-769.

https://doi.org/10.1016/j.eururo.2012.12.054

Chen YA, Chen KC, Tsai VF, Huang ZW, Hsieh JT, Wo AM. Direct Characterization of Motion-Dependent Parameters of Sperm in a Microfluidic Device: Proof of Principle. Clin Chem 2013; 59: 493-501.

https://doi.org/10.1373/clinchem.2012.190686

Cardona Maya W. Re: High-throughput lensfree 3D tracking of human sperms reveals rare statistics of helical trajectories. European Urology 2013: 768-769

https://doi.org/10.1016/j.eururo.2012.12.054

Cardona Maya W. Análisis cuantitativo del movimiento de espermatozoides humanos aplicando un programa de uso libre, estudio-piloto. Rev UDCA Act & Div Cient 2013; 16: 313-317.

Verstegen J, Iguer-Ouada M, Onclin K. Computer assisted semen analyzers in andrology research and veterinary practice. Theriogenology 2002; 57: 149-179.

https://doi.org/10.1016/S0093-691X(01)00664-1

Taha EA, Ez-Aldin AM, Sayed SK, Ghandour NM, Mostafa T. Effect of smoking on sperm vitality, DNA integrity, seminal oxidative stress, zinc in fertile men. Urology 2012; 80: 822-825.

https://doi.org/10.1016/j.urology.2012.07.002

Givan AL. Flow cytometry. Flow Cytometry Protocols: Springer; 2004: 1-31.

Hossain MS, Johannisson A, Wallgren M, Nagy S, Siqueira AP, Rodriguez-Martinez H. Flow cytometry for the assessment of animal sperm integrity and functionality: state of the art. Asian J Androl 2011; 13: 406-419.

https://doi.org/10.1038/aja.2011.15

Cardona-Maya W, Cadavid A. Evaluación de la reacción acrosomal en espermatozoides humanos inducida por los monosacáridos manosa y N-acetilglucosamina. Actas Urol Esp 2005; 29: 676-684.

https://doi.org/10.1016/S0210-4806(05)73318-0

Berridge MV, Herst PM, Tan AS. Tetrazolium dyes as tools in cell biology: new insights into their cellular reduction. Biotechnol Annu Rev 2005; 11: 127-152.

https://doi.org/10.1016/S1387-2656(05)11004-7

Aziz DM. Assessment of bovine sperm viability by MTT reduction assay. Anim Reprod Sci 2006; 92: 1-8.

https://doi.org/10.1016/j.anireprosci.2005.05.029

Ospina Medina L, Álvarez Gómez Á, Arango Valencia V, Cadavid Jaramillo Á, Cardona Maya W. Actividad espermicida y citotóxica del extracto de Sapindus saponaria L.(jaboncillo). Rev Cubana Plant Med 2013; 18: 187-200.

Pitnick S, Hosken DJ, Birkhead TR. 3 Sperm morphological diversity. Sperm biology: an evolutionary perspective 2008: 69.

https://doi.org/10.1016/B978-0-12-372568-4.00003-3

Verstegen J, Iguer-Ouada M, Onclin K. Computer assisted semen analyzers in andrology research and veterinary practice. Theriogenology 2002; 57: 149-179.

https://doi.org/10.1016/S0093-691X(01)00664-1

Kashou AH, Sharma R, Agarwal A. Assessment of oxidative stress in sperm and semen. Methods Mol Biol 2013; 927: 351-361.

https://doi.org/10.1007/978-1-62703-038-0_30

Aziz N, Saleh RA, Sharma RK, Lewis-Jones I, Esfandiari N, Thomas Jr AJ, et al. Novel association between sperm reactive oxygen species production, sperm morphological defects, and the sperm deformity index. Fertil Steril 2004; 81: 349-354.

https://doi.org/10.1016/j.fertnstert.2003.06.026

Mahfouz R, Sharma R, Lackner J, Aziz N, Agarwal A. Evaluation of chemiluminescence and flow cytometry as tools in assessing production of hydrogen peroxide and superoxide anion in human spermatozoa. Fertil Steril 2009; 92: 819-827.

https://doi.org/10.1016/j.fertnstert.2008.05.087

Gil-Villa AM, Cardona-Maya W, Agarwal A, Sharma R, Cadavid Á. Role of male factor in early recurrent embryo loss: do antioxidants have any effect? Fertil Steril 2009; 92: 565-571.

https://doi.org/10.1016/j.fertnstert.2008.07.1715

Aitken RJ, Baker MA, O'Bryan M. Shedding light on chemiluminescence: the application of chemiluminescence in diagnostic andrology. J Androl 2004; 25: 455-465.

https://doi.org/10.1002/j.1939-4640.2004.tb02815.x

Baker MA, Aitken RJ. Reactive oxygen species in spermatozoa: methods for monitoring and significance for the origins of genetic disease and infertility. Reprod Biol Endocrinol 2005; 3: 67.

https://doi.org/10.1186/1477-7827-3-67

McKinney K, Lewis S, Thompson W. Reactive oxygen species generation in human sperm: luminol and lucigenin chemiluminescence probes. Syst Biol Reprod Med 1996; 36: 119-125.

https://doi.org/10.3109/01485019608987087

Soh N. Recent advances in fluorescent probes for the detection of reactive oxygen species. Anal Bioanal Chem 2006; 386: 532-543.

https://doi.org/10.1007/s00216-006-0366-9

Stowe DF, Camara AK. Mitochondrial reactive oxygen species production in excitable cells: modulators of mitochondrial and cell function. Antioxid Redox Signal 2009; 11: 1373-1414.

https://doi.org/10.1089/ars.2008.2331

Virro MR, Larson-Cook KL, Evenson DP. Sperm chromatin structure assay (SCSA®) parameters are related to fertilization, blastocyst development, and ongoing pregnancy in in vitro fertilization and intracytoplasmic sperm injection cycles. Fertil Steril 2004; 81: 1289-1295.

https://doi.org/10.1016/j.fertnstert.2003.09.063

Simon L, Brunborg G, Stevenson M, Lutton D, McManus J, Lewis SE. Clinical significance of sperm DNA damage in assisted reproduction outcome. Hum Reprod 2010; 25: 1594-1608.

https://doi.org/10.1093/humrep/deq103

Sakkas D, Alvarez JG. Sperm DNA fragmentation: mechanisms of origin, impact on reproductive outcome, and analysis. Fertil Steril 2010; 93: 1027-1036.

https://doi.org/10.1016/j.fertnstert.2009.10.046

Rouen A, Pyram K, Pollet-Villard X, Hyon C, Dorna M, Marques S, et al. Simultaneous cell by cell study of both DNA fragmentation and chromosomal segregation in spermatozoa from chromosomal rearrangement carriers. J Assist Reprod Genet 2013; 30: 383-390.

https://doi.org/10.1007/s10815-012-9915-7

Lewis S, Aitken R. DNA damage to spermatozoa has impacts on fertilization and pregnancy. Cell Tissue Res 2005; 322: 33-41.

https://doi.org/10.1007/s00441-005-1097-5

Perreault SD, Aitken RJ, Baker HG, Evenson DP, Huszar G, Irvine DS, et al. Integrating new tests of sperm genetic integrity into semen analysis: breakout group discussion. Advances in Male Mediated Developmental Toxicity: Springer; 2003: 253-268.

https://doi.org/10.1007/978-1-4419-9190-4_23

McPherson SM, Longo FJ. Nicking of rat spermatid and spermatozoa DNA: possible involvement of DNA topoisomerase II. Dev Biol 1993; 158: 122-130.

https://doi.org/10.1006/dbio.1993.1173

Aoki VW, Liu L, Carrell DT. Identification and evaluation of a novel sperm protamine abnormality in a population of infertile males. Hum Reprod 2005; 20: 1298-1306.

https://doi.org/10.1093/humrep/deh798

Sakkas D, Manicardi G, Bianchi PG, Bizzaro D, Bianchi U. Relationship between the presence of endogenous nicks and sperm chromatin packaging in maturing and fertilizing mouse spermatozoa. Biol Reprod 1995; 52: 1149-1155.

https://doi.org/10.1095/biolreprod52.5.1149

Smith TB, Dun MD, Smith ND, Curry BJ, Connaughton HS, Aitken RJ. The presence of a truncated base excision repair pathway in human spermatozoa that is mediated by OGG1. J Cell Sci 2013; 126: 1488-1497.

https://doi.org/10.1242/jcs.121657

Hammadeh ME, al-Hasani S, Stieber M, Rosenbaum P, Kupker D, Diedrich K, et al. The effect of chromatin condensation (aniline blue staining) and morphology (strict criteria) of human spermatozoa on fertilization, cleavage and pregnancy rates in an intracytoplasmic sperm injection programme. Hum Reprod 1996; 11: 2468-2471.

https://doi.org/10.1093/oxfordjournals.humrep.a019139

Singh NP, McCoy MT, Tice RR, Schneider EL. A simple technique for quantitation of low levels of DNA damage in individual cells. Exp Cell Res 1988; 175: 184-191.

https://doi.org/10.1016/0014-4827(88)90265-0

Singh NP, Muller CH, Berger RE. Effects of age on DNA double-strand breaks and apoptosis in human sperm. Fertil Steril 2003; 80: 1420-1430.

https://doi.org/10.1016/j.fertnstert.2003.04.002

Gorczyca W, Traganos F, Jesionowska H, Darzynkiewicz Z. Presence of DNA Strand Breaks and Increased Sensitivity of DNA in Situ to Denaturation in Abnormal Human Sperm Cells: Analogy to Apoptosis of Somatic Cells. Exp Cell Res 1993; 207: 202-205.

https://doi.org/10.1006/excr.1993.1182

Sakkas D, Moffatt O, Manicardi GC, Mariethoz E, Tarozzi N, Bizzaro D. Nature of DNA damage in ejaculated human spermatozoa and the possible involvement of apoptosis. Biol Reprod 2002; 66: 1061-1067.

https://doi.org/10.1095/biolreprod66.4.1061

Evenson D, Jost L, Marshall D, Zinaman M, Clegg E, Purvis K, et al. Utility of the sperm chromatin structure assay as a diagnostic and prognostic tool in the human fertility clinic. Hum Reprod 1999; 14: 1039-1049.

https://doi.org/10.1093/humrep/14.4.1039

Mayorga-Torres B, Cardona-Maya W, Cadavid A, Camargo M. Evaluation of sperm functional parameters in normozoospermic infertile individuals. Actas Urol Esp 2013; 37: 221-227.

https://doi.org/10.1016/j.acuro.2012.06.008

Agarwal A, Said TM. Role of sperm chromatin abnormalities and DNA damage in male infertility. Hum Reprod Update 2003; 9: 331-345.

https://doi.org/10.1093/humupd/dmg027

Huszar G, Sbracia M, Vigue L, Miller DJ, Shur BD. Sperm plasma membrane remodeling during spermiogenetic maturation in men: relationship among plasma membrane beta 1, 4-galactosyltransferase, cytoplasmic creatine phosphokinase, and creatine phosphokinase isoform ratios. Biol Reprod 1997; 56: 1020-1024.

https://doi.org/10.1095/biolreprod56.4.1020

Aziz N, Agarwal A. Evaluation of sperm damage: beyond the World Health Organization criteria. Fertil Steril 2008; 90: 484-485.

https://doi.org/10.1016/j.fertnstert.2007.07.1287

Cardona Maya W. Manual de procesamiento de semen humano de la Organización Mundial de la Salud-2010. Actas Urol Esp 2010; 34: 577-578.

https://doi.org/10.1016/j.acuro.2010.05.002

Cunningham RE. Overview of flow cytometry and fluorescent probes for cytometry. Methods Mol Biol 1999; 115: 249-256.

https://doi.org/10.1385/1-59259-213-9:249

Evenson DP. Flow cytometric analysis of male germ cell quality. Methods Cell Biol 1990; 33: 401-410.

https://doi.org/10.1016/S0091-679X(08)60543-9

Evenson DP, Darzynkiewicz Z, Melamed MR. Comparison of human and mouse sperm chromatin structure by flow cytometry. Chromosoma 1980; 78: 225-238.

https://doi.org/10.1007/BF00328394

Cómo citar
1.
Puerta-Suárez J, Mayorga-Torres JM, Ospina-Medina L, Berdugo-Gutiérrez J, Cardona-Maya W. La calidad espermática evaluada mediante metodologías no convencionales. Med. Lab. [Internet]. 1 de marzo de 2014 [citado 28 de junio de 2022];20(3-4):153-68. Disponible en: https://medicinaylaboratorio.com/index.php/myl/article/view/168
Publicado
2014-03-01
Sección
Salud sexual y reproductiva

Artículos más leídos del mismo autor/a