Background: Dry eye disease is a multifactorial disease of ocular surface characterized by loss of homeostasis of the tear film leading to ocular discomfort, visual disturbances, ocular surface inflammation and damage. Ocular and systemic medications are important risk factors for Dry eye disease. Glaucoma, generally affecting people aged 40 and above are widely treated with topical hypotensive agents for long duration increasing the risk of Dry eye disease. Previous studies have shown correlation between anti glaucoma medications and Dry eye disease. This study aims to assess severity of dry eye disease among glaucoma patients on topical hypotensive medication. Methods: A cross-sectional study on 160 glaucoma patients and 160 controls were conducted in the Ophthalmology department of a Tertiary care centre in Eastern India. Institutional Ethical Committee approval and informed consent of participants were taken. OSDI questionnaire, Schirmer test and Tear film break up time of each participant were used for analysing data. Results: This study comprised participants of age group 61-70 yrs. Mild to severe levels of OSDI value was detected in122(76%) glaucoma patients and 106 (66%) controls. Abnormal Schirmer Test was found in 57 % of glaucoma patients and 52% of controls. Tear film break up time was abnormal in 79(49%) glaucoma patients and 72(45%) control with a p value of 0.02 which was found to be significant. Conclusion: Dry eye disease is highly prevelant in Glaucoma patients and often triggered or aggravated by long term usage of topical anti glaucoma drugs. Patient awareness may contribute to reduced non compliance of medications and ultimately improve prognosis and preservation of vision.